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KMID : 0614020000160010044
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Journal of Pharmaceutical Sciences (C.N.U.)
2000 Volume.16 No. 1 p.44 ~ p.51
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Effect of Endogenous Detergents on Enzymatic Release of 5’-Nucleotidase from Ox Brain Membrane
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Liu-Xi-Wen
Sok Dai-Eun
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Abstract
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Glycosylphosphatidylinositol (GPI)-linked 5’-nucleotidase is released as both amphiphilic form (Amp-nucleotidase) and hydrophilic form (Hyd-nucleotidase) from bovine brain membrane. Exposure of brain membrane to deoxycholate, lysolecithin or monooleoylglycerol leads to a concentration-dependent release of Hyd-nucleotidase with deoxycholate being the most effective. Next, the brain GPI-PLD-catalyzed conversion of Amp-nucleotidase to Hyd-nucleotidase was investigated. The GPI-PLD-catalyzed conversion of required detergents, and among detergents used, monooleoylglycerol was the most effective for the enzymatic conversion. Monooleoylglycerol exhibited a concentration-dependent effect on GPI-PLD activity up to 0.3 mM, but its effect decreased at 1 mM. In addition, the combinational effect of monooleoylglycerol and lysolecithin on GPI-PLD action was not significant. Based on these results. it is suggested that the formation of Hyd-nucleotidase in brain tissue may be ascribed to the activities of GPI-PLC and GPI-PLD in the presence of bio-detergent.
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KEYWORD
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5’-Nucleotidase, glycosylphosphatidylinositol, amphiphilic, GPI-PLD
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